For investigate reasons, RT‐qPCR is often used not only to ascertain the presence or absence of an RNA but to evaluate its concentration. In such cases, a calibration curve must be A part of just about every experiment, consisting of a minimum of six serial dilutions of the in vitro−transcribed https://www.quora.com/profile/Even-Sa/How-to-use-your-nucleic-acid-extraction-reagents-correctly-Each-kit-comes-with-a-detailed-instruction-manual-includin