5-mL tubes from the magnetic stand for 5 min, then clear away the buffer using a pipette and wash the beads 2× with five hundred μL of refreshing 80% ethanol. Soon after the 2nd wash, take out all ethanol and Be certain that no ethanol is remaining during the samples. https://rnabeads03703.newsbloger.com/30178420/isolation-of-trace-rna-fundamentals-explained